什么是ELISA微孔板？一文带您了解类型、规格和应用

<p>&nbsp;<font face="宋体" style="font-size: 10.5pt;">　　酶联免疫吸附实验（</font><span style="font-family: Calibri; font-size: 10.5pt;">ELISA</span><font face="宋体" style="font-size: 10.5pt;">）是一种常用的基于板的免疫学领域金标准测定法，用于生命科学和分子生物学实验检测和量化复杂混合物中的生物分子。</font></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　在技术上，将抗原或抗体包被在</font>96<font face="宋体">或</font><font face="Calibri">384ELISA</font><font face="宋体">微孔板的表面。然后，将微孔板与抗原特异性抗体一起孵育，这些抗体会与微孔板底部目标抗原结合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>ELISA<font face="宋体">板是</font><font face="Calibri">ELISA</font><font face="宋体">检测的核心。它们也被称为微量滴定板或微孔板，由聚苯乙烯或聚丙烯制成。如今，市场上有各种</font><font face="Calibri">ELISA</font><font face="宋体">板或微孔板可供选择，以适应不同的实验室工作流程。因此，要开发精确、准确和灵敏的生物测定，选择合适的板至关重要，从而选择合适的表面来固定目标生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　在本文中，酶联生物将为您介绍下</font>ELISA<font face="宋体">微孔板特点、市场上可买到的微孔板的格式及其多种应用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>Elisa<font face="宋体">板类型</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　微孔板有多种类型，每种类型都针对特定的检测要求和检测方法量身定制。每个微孔板的表面都对特定生物分子具有高结合亲和力。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　亲和免疫分析板</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　亲和免疫测定板是为了增强抗原和抗体的结合而开发的。它为固定生物分子提供了高亲和力的表面，从而提高了测定灵敏度、定量准确度和最佳相互作用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　这些板用于</font>ELISA<font face="宋体">和免疫测定。它们涂有镍、链霉亲和素、抗体和其他配体。它有多种类型，包括：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　生物素结合板：包括</font>NeutrAvidin<font face="宋体">包被板和链霉亲和素包被板。这些板通常用于以生物素化分子（如抗体或核酸）作为探针或检测剂的检测。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　抗体结合板：抗体结合板的类型包括蛋白</font>A/G/L<font face="宋体">包被板和二抗（</font><font face="Calibri">GAM</font><font face="宋体">、</font><font face="Calibri">GAR</font><font face="宋体">）包被板。它们与各种类型的免疫球蛋白（</font><font face="Calibri">IgG</font><font face="宋体">、</font><font face="Calibri">IgM</font><font face="宋体">和</font><font face="Calibri">IgA</font><font face="宋体">等）和一抗结合，用于高效抗原检测。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>His<font face="宋体">标签结合板：镍和铜涂层板是</font><font face="Calibri">His</font><font face="宋体">标签结合板的几种类型。它们广泛用于涉及组氨酸标记蛋白的测定。</font><font face="Calibri">His</font><font face="宋体">标签结合板可实现</font><font face="Calibri">His</font><font face="宋体">标签蛋白的特异性固定和检测。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>GST<font face="宋体">结合板：包括谷胱甘肽</font><font face="Calibri">(GSH)</font><font face="宋体">涂层板，用于检测和分析</font><font face="Calibri">GST</font><font face="宋体">标记或谷胱甘肽</font><font face="Calibri">S-</font><font face="宋体">转移酶</font><font face="Calibri">(GST)</font><font face="宋体">融合蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font><strong><font face="宋体">吸收免疫分析板</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　吸收免疫测定板也称为结合免疫测定板。这些板是为蛋白质、抗体和其他生物分子的被动吸附（包被）而开发的。它们被设计为专门针对特点的抗原。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　它也有两种类型：</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　亲水性蛋白质结合板：它们旨在吸收亲水性的生物分子，例如糖蛋白、抗体、聚糖和其他可溶性蛋白质。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　疏水性脂质结合板：它们被设计用于吸收疏水性的生物分子，例如脂质和脂蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　<strong>共价免疫分析板</strong></font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　这些专门设计的微孔板可实现肽、核酸和其他生物分子的共价连接。它们能够与各种功能基团结合，包括氨基</font>(NH2)<font face="宋体">、巯基</font><font face="Calibri">(SH)</font><font face="宋体">、羧基</font><font face="Calibri">(COOH)</font><font face="宋体">和磷酸基</font><font face="Calibri">(PO4)</font><font face="宋体">，从而实现稳定而高效的固定。它们可用于</font><font face="Calibri">ELISA</font><font face="宋体">和其他基于板的检测技术。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　共价免疫测定板的一个例子是核酸测定板。它们有多种类型，以适应寡核苷酸与各种功能基团或标签的结合。这些板可以通过共价键或亲和相互作用实现牢固连接，确保在实验中准确可靠地分析核酸。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>Elisa<font face="宋体">板工作原理</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>ELISA<font face="宋体">板是免疫学和诊断实验室中定期进行</font><font face="Calibri">ELISA</font><font face="宋体">检测的必备材料。它确保目标抗原的结合并保持目标分析物和抗体之间的化学反应。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　为了开发有效的免疫测定法，用适当的蛋白质或抗体包被</font>ELISA<font face="宋体">板至关重要。根据所需的检测信号选择合适的微孔板类型对于实现最佳的板包被至关重要。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　检测微孔板颜色的选择取决于所检测的信号。例如，黑色或白色不透明板用于检测化学发光和荧光信号，而透明聚苯乙烯平底板用于检测比色信号。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　确保板上没有划痕，以免干扰分析和最终结果。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　包被过程涉及被动吸附，即将蛋白质或抗体添加到板中，并让其与塑料表面结合。通常，先制备蛋白质或抗体溶液，然后在板上孵育特定时间。孵育后，除去包被溶液，并添加封闭缓冲液以防止非特异性结合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　需要注意的是，理想包被条件和结合能力可能因所用的特定蛋白质或抗体而异。通常需要通过实验来确定适当的包被浓度。包被板的蛋白质含量略高于所需量可实现更广泛的检测范围，但包被量过大可能导致非特异性结合和高背景信号。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　板子包被后，可立即使用或储存在</font>4&deg;C<font face="宋体">以备将来使用，具体取决于包被蛋白的稳定性。适当的储存条件可确保检测结果的可靠性和可重复性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>ELISA<font face="宋体">板用途</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>ELISA<font face="宋体">聚苯乙烯微孔板在研究、临床和诊断实验室及行业中有着广泛的应用。它使科研人员能够量化样本中目标分子的存在和浓度，例如血液、血清、血浆、尿液或细胞裂解物。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　这些板具有多个孔或隔间，可同时评估多个样品或测试条件。它们可进行高通量筛选，使研究人员能够高效处理多个样品。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>ELISA<font face="宋体">板兼容各种检测方法，包括比色、荧光和化学发光技术，为检测和测量感兴趣的抗原提供了灵活性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font><strong>ELISA<font face="宋体">检测</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　</font>ELISA<font face="宋体">是实验室中最常见和最广泛使用的基于板的免疫测定技术之一。除了微量离心管和移液器外，该测定还需要</font><font face="Calibri">8</font><font face="宋体">孔条、</font><font face="Calibri">96</font><font face="宋体">孔或</font><font face="Calibri">384</font><font face="宋体">孔</font><font face="Calibri">ELISA</font><font face="宋体">中结合或高结合板。它们允许同时评估多种生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　对于从事组织培养或基于细胞的分析的研究人员来说，介质结合</font>ELISA<font face="宋体">板为细胞粘附和生长提供了最佳表面。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　孵育和清洗培养板时，必须正确密封培养板，以防止交叉污染并保持样品的完整性。培养板密封器可以简化此过程。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　使用</font>ELISA<font face="宋体">板时，请考虑用于产生信号的底物。不同的底物提供各种检测选项，包括比色和化学发光信号，让您可以根据实验需要选择最合适的方法。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋体">　　以上就是关于</font>ELISA<font face="宋体">微孔板的介绍。选择合适的微孔板对</font><font face="Calibri">ELISA</font><font face="宋体">实验结果至关重要，建议根据实验需求，例如结合类型和材质，仔细选择合适的</font><font face="Calibri">ELISA</font><font face="宋体">微孔板，以确保实验的成功。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋体;&#10;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>