ELISA试剂盒实验的温育方法

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">实验中通常有两次抗原抗体反响，即加标本和加酶联络物后。抗原抗体反响的完毕需求有必定的温度和时刻，这一保温进程称为温育</font><font face="Calibri">(incubation)</font><font face="宋体">，有人称之为孵育，在</font><font face="Calibri">ELISA</font><font face="宋体">中似不恰当。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">属固相免疫测定，抗原、抗体的联络只在固相表面上发生。以抗体包被的夹心法为例，参加板孔中的标本，其间的抗原并不是都有对等的和固相抗联络的时机，只需最贴近孔壁的一层溶液中的抗原直接与抗体触摸。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">这是一个逐渐平衡的进程，因此需经涣散才调抵达反响的完毕。在这今后参加的酶符号抗体与固相抗原的联络也相同如此。这便是为何</font>ELISA<font face="宋体">反响老是需求必定时刻的温育。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">温育常选用的温度有</font>43<font face="宋体">℃、</font><font face="Calibri">37</font><font face="宋体">℃、室温文</font><font face="Calibri">4</font><font face="宋体">℃（冰箱温度）等。</font><font face="Calibri">37</font><font face="宋体">℃是实验室中常用的保温温度，也是大多数抗原抗体联络的合适温度。在建立</font><font face="Calibri">ELISA</font><font face="宋体">方法作反响动力学研讨时，实验标明，两次抗原抗体反响通常在</font><font face="Calibri">37</font><font face="宋体">℃经</font><font face="Calibri">1-2</font><font face="宋体">小时，产品的生成可达高峰。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">为加快反响，可前进反响的温度，有些实验在</font>43<font face="宋体">℃进行，但不宜选用更高的温度。抗原抗体反响</font><font face="Calibri">4</font><font face="宋体">℃更为彻底，在放射免疫测定中多使反响在冰箱中过夜，以构成最多的堆积。但因所需时刻太长，在</font><font face="Calibri">ELISA</font><font face="宋体">中通常不予选用。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋体">试剂盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">保温的方法除有的</font><font face="Calibri">ELISA</font><font face="宋体">仪器附有特制的电热块外，通常均选用水浴，可将</font><font face="Calibri">ELISA</font><font face="宋体">板置于水浴箱中，</font><font face="Calibri">ELISA</font><font face="宋体">板底应贴着水面，使温度活络平衡。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">为防止蒸发，板上应加盖，也可用塑料贴封纸或保鲜膜覆盖板孔，此时可让反响板漂浮在水面上。若用保温箱，</font>ELISA<font face="宋体">板应放在湿盒内，湿盒要选用传热性杰出的资料如金属等，在盒底垫湿的纱布，最终将</font><font face="Calibri">ELISA</font><font face="宋体">板放在湿纱布上。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">湿盒应先放在保温箱中预温至规则的温度，特别是在气温较低的时分更应如此。无论是水浴仍是湿盒温育，反响板均不宜叠放，以确保各板的温度都能活络平衡。室温温育的反响，操作时的室温应严重束缚在规则的范围内，标准室温温度是指</font>20-25<font face="宋体">℃，但具体操作时可根据说明书的央求操控温育。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋体">室温温育时，</font>ELISA<font face="宋体">板只需平置于操作台上即可。应留神温育的温度和时刻应按规则力求精确。为确保这一点，一个人操作时，一次不宜多于两块板一同测定。</font></span><span style="mso-spacerun:'yes';font-family:宋体;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>