<div>
<div><strong>植物种子蛋白提取试剂盒</strong>提供全套试剂,适用于从各种高淀粉含量的 植物种子样品中提取可溶性蛋白。如水稻、荞麦、玉米、小麦等。提取过程简单方便。</div>
<div> </div>
</div>
<div><strong>本试剂盒</strong>不可用于诊断或治疗用途!使用产品时,请参照试剂盒中随产品附带的 ,需要最新电子版说明书时可以在收到产品后发邮件索取。使用时需要合适的实验室外套, 一次性手套。避免皮肤或粘膜与试剂接触。如果试剂不小心接触皮肤或眼睛,应立即用水冲洗。</div>
<div><strong><br />
试剂盒组成</strong><strong>: </strong></div>
<div>
<table class="MsoTableGrid" border="1" cellspacing="0" style="border-collapse:collapse;width:383.9000pt;margin-left:6.7500pt; margin-right:6.7500pt;border:none;mso-border-left-alt:0.5000pt solid windowtext; mso-border-top-alt:0.5000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext;mso-border-bottom-alt:0.5000pt solid windowtext; mso-border-insideh:0.5000pt solid windowtext;mso-border-insidev:0.5000pt solid windowtext;mso-padding-alt:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;">
<tbody>
<tr style="height:27.4000pt;">
<td width="178" valign="center" style="width:134.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:1.0000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;background:rgb(242,186,2);">
<p class="MsoNormal"><span style="font-size: x-small;"><b><span style="font-family: 微软雅黑;">产品组成</span></b></span><b><span style="font-family: 微软雅黑; font-size: 10.5pt;"><o:p></o:p></span></b></p>
</td>
<td width="86" valign="center" style="width:65.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:1.0000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;background:rgb(242,186,2);">
<p class="MsoNormal"><span style="font-size: x-small;"><b><span style="font-family: 微软雅黑;">50T</span></b></span><b><span style="font-family: 微软雅黑; font-size: 10.5pt;"><o:p></o:p></span></b></p>
</td>
<td width="92" valign="center" style="width:69.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:1.0000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;background:rgb(242,186,2);">
<p class="MsoNormal"><span style="font-size: x-small;"><b><span style="font-family: 微软雅黑;">100T</span></b></span><b><span style="font-family: 微软雅黑; font-size: 10.5pt;"><o:p></o:p></span></b></p>
</td>
<td width="153" valign="center" style="width:115.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:1.0000pt solid windowtext;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;background:rgb(242,186,2);">
<p class="MsoNormal"><span style="font-size: x-small;"><b><span style="font-family: 微软雅黑;">储存条件</span></b></span><b><span style="font-family: 微软雅黑; font-size: 10.5pt;"><o:p></o:p></span></b></p>
</td>
</tr>
<tr style="height:24.9000pt;">
<td width="178" valign="center" style="width:134.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">组份</span><span style="font-family: 微软雅黑;">A: </span><span style="font-family: 微软雅黑;"><font face="微软雅黑">蛋白提取液</font> <font face="微软雅黑">A</font></span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="86" valign="center" style="width:65.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">50 ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="92" valign="center" style="width:69.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">100ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="153" valign="center" style="width:115.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">2-8℃保存</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
</tr>
<tr style="height:31.2500pt;">
<td width="178" valign="center" style="width:134.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">组份</span><span style="font-family: 微软雅黑;">B: </span><span style="font-family: 微软雅黑;"><font face="微软雅黑">蛋白提取液</font> <font face="微软雅黑">B</font></span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="86" valign="center" style="width:65.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">5 ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="92" valign="center" style="width:69.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">10ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="153" valign="center" style="width:115.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">2-8℃保存</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
</tr>
<tr style="height:31.2500pt;">
<td width="178" valign="center" style="width:134.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">组份</span><span style="font-family: 微软雅黑;">C: </span><span style="font-family: 微软雅黑;"><font face="微软雅黑">蛋白提取液</font> <font face="微软雅黑">C</font></span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="86" valign="center" style="width:65.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">10 ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="92" valign="center" style="width:69.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">20ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="153" valign="center" style="width:115.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">2-8℃保存</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
</tr>
<tr style="height:31.7000pt;">
<td width="178" valign="center" style="width:134.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">组份</span><span style="font-family: 微软雅黑;">D: </span><span style="font-family: 微软雅黑;"><font face="微软雅黑">蛋白提取液</font> <font face="微软雅黑">D</font></span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="86" valign="center" style="width:65.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">5 ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="92" valign="center" style="width:69.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">10ml</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
<td width="153" valign="center" style="width:115.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt ;border-left:1.0000pt solid windowtext; mso-border-left-alt:0.5000pt solid windowtext;border-right:1.0000pt solid windowtext;mso-border-right-alt:0.5000pt solid windowtext; border-top:none;mso-border-top-alt:0.5000pt solid windowtext;border-bottom:1.0000pt solid windowtext; mso-border-bottom-alt:0.5000pt solid windowtext;">
<p class="MsoNormal"><span style="font-size: x-small;"><span style="font-family: 微软雅黑;">2-8℃保存</span></span><span style="font-family:微软雅黑;font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
</td>
</tr>
</tbody>
</table>
</div>
<div><strong>注意事项: </strong> </div>
<div>1.蛋白酶抑制剂未开盖使用前也可以 2-8℃储存。开盖使用后-20℃储存。</div>
<div>2.蛋白酶抑制剂在 2-8℃低温时是固体状态,从冰箱取出后恢复至室温或 37℃短时间水 浴,变成液体状态后离心至管底部再开盖。</div>
<div>3.试剂拆封后请尽快使用完!</div>
<div><strong><br />
仪器准备</strong><strong>:</strong><br />
1.离心机</div>
<div>2.振荡器</div>
<div>3.匀浆机/匀浆器</div>
<div>4.涡旋混匀器</div>
<div>5.移液器</div>
<div>6.冰箱</div>
<div>7.冰盒</div>
<div><strong><br />
试剂准备:</strong><br />
1.PBS缓冲液</div>
<div>2.蛋白定量试剂盒</div>
<div><strong><br />
耗材准备:</strong><br />
1.离心管</div>
<div>2.吸头</div>
<div>3.一次性手套</div>
用户评论(共6条评论)
酶联生物经过不断的实验优化和改进,积累了大量的经验,拥有专业的酶联研发团队。利用专业的酶联免疫技术自主研发的elisa试剂盒,能对血清及其它样本定量检测抗原,定性检测特异性抗体。优质的试剂,先进的仪器和正确的操作是保证ELISA检测结果准确可靠的必要条件。ELISA检测的方便性、稳定性、重复性和可靠性方面都具有很大的优势。
ELISA检测技术服务内容:
1、双抗体夹心法检测抗原 2、间接法检测抗体 3、为客户提供各种ELISA技术进行样本检测。
以上代测费,凡购买本公司试剂盒,我们免费代测!
凡购买本公司目录任何一种酶联免疫检测试剂盒,您只需将需要检测的动物(Human, Rat, Mouse, Rabbit, Monkey,
Pig……)种类和检测指标(白介素类、激素类)及标本数量(48T/96T)通知公司业务员即可。在接到客户标本当日起,现货产品一周内将检测报告交到客户手中!
欢迎各科研单位在各种项目上与我们公司开展不同层次的密切合作,以双赢求发展,共同进步,为中国检测事业的发展积累经验。
二、样本要求
在收集标本前都必须有一个完整的计划,必须清楚要检测的成份是否足够稳定。我们提倡新鲜标本尽早检测,对收集后当天就进行检测的标本,及时储存在4℃备用,如有特殊原因需要周期收集标本,请造模取材后,将标本及时分装后放在-20℃或-70℃条件下保存。因冰室与室温存在一定温差,蛋白极易降解,直接影响实验质量,所以避免反复冻融。代测放免标本的客户取材前须向我司销售人员索要说明书,具体操作注意事项请与我司技术人员沟通。
液体类标本:标本必须为液体,不含沉淀。包括血清、血浆、尿液、胸腹水、脑脊液、细胞培养上清、组织匀浆等。
血清:室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。收集上清。如有沉淀形成,应再次离心。
血浆:应根据试剂盒的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,加入10%(v/v)抗凝剂(0.1M柠檬酸钠或1%heparin
或2.0%EDTA.Na2)混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。如有沉淀形成,应再次离心。
尿液、胸腹水、脑脊液:用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。如有沉淀形成,应再次离心。
细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.0-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
组织标本:切割标本后,称取重量。加入一定量的PBS,缓冲液中可加入1μg/L蛋白酶抑制剂或50U/ml的Aprotinin(抑肽酶)。用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清置于-20度或-70度保存,如有必要,可以将样品浓缩干燥。分装后一份待检测,其余冷冻备用。
三、寄标本时需注明以下情况:
1、标本编号;2、所测项目;3、是否做复孔;3、联系方式;4、实验后标本是否寄回。
客户须知:
客户应对所提供的材料及信息负责,如因客户提供的材料及信息不准确而引起的实验延误或经济损失由客户承担。
ELISA试剂盒实验中的常见问题
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无信号
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可能原因
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解决方法
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试剂添加顺序错误或试剂配制有误
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重复实验
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检查试剂配制方法
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复核试验试剂添加流程
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HRP被叠氮化合物污染
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用新鲜配制的试剂
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体用量不足
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使用推荐的抗体量
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边缘效应
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可能原因
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解决方法
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孵育温度不均衡
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孵育时使用封板胶纸
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勿在环境温度变化大的地方孵育
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显示缓慢
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可能原因
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解决方法
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酶标板未处于正确温度下
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确保使用前酶标板和试剂处于室温下
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溶液污染
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避免使用含NaN3的试剂
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高背景信号
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可能原因
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解决方法
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洗板不充分
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洗涤缓冲液加入孔中洗涤,确保洗涤充分
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确保在洗涤前弃掉了所有残留抗体溶液
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样品或标准品中含干扰物
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设置空白对照
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冲液受污染
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重新配制缓冲液
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板间重复性差
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可能原因
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解决方法
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洗板不充分
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将洗涤缓冲液加入孔中洗涤,确保洗涤充分
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确保已在洗涤前弃掉所有残留溶液
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孵育温度发生变化
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严格按照推荐的温度孵育
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勿在环境温度变化大的地方孵育
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操作流程改变
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严格按照相同的操作流程
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封板胶纸重复使用导致HRP残留污染
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每一步均使用新的封板胶纸
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整版读数偏低
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可能原因
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解决方法
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酶标仪波长设置不正确
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检查酶标仪参数设置
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包被的酶标板超过使用效期
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更换新批次试剂盒
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