<div><br />
中文名称:<span style="color: rgb(255, 153, 0);"><strong>兔抗SLC3A2多克隆抗体</strong></span> </div>
<div><br />
英文名称:Anti-SLC3A2 rabbit polyclonal antibody</div>
<div><br />
别 名:solute carrier family 3 member 2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC</div>
<div><br />
<span style="color: rgb(255, 153, 0);"><strong>相关类别:一抗</strong></span></div>
<div><br />
储 存:冷冻(-20℃)</div>
<div><br />
宿 主:Rabbit</div>
<div> </div>
<div>抗 原:SLC3A2</div>
<div><br />
反应种属:Human</div>
<div><br />
标记物:Unconjugate</div>
<div><br />
克隆类型:rabbit polyclonal</div>
<div><br />
技术规格<br />
</div>
<table class="MsoNormalTable" border="1" cellspacing="0" style="border-collapse:collapse;width:422.8000pt;margin-left:1.6500pt;mso-table-layout-alt:fixed;border:none;mso-border-left-alt:0.7500pt solid rgb(197,202,208);mso-border-top-alt:0.7500pt solid rgb(197,202,208);mso-border-right-alt:0.7500pt solid rgb(197,202,208);mso-border-bottom-alt:0.7500pt solid rgb(197,202,208);mso-padding-alt:0.0000pt 0.0000pt 0.0000pt 0.0000pt ;">
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-width: 1pt; border-style: dotted; border-color: rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Background:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: 1pt dotted rgb(193, 193, 193); border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Applications:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">ELISA, WB, IHC</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Name of antibody:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">SLC3A2</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Immunogen:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Fusion protein of human SLC3A2</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Full name:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">solute carrier family 3 member 2</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Synonyms:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">SwissProt:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">P08195</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">ELISA Recommended dilution:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">5000-10000</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">IHC positive control:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">Human colorectal cancer and Human liver cancer</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">IHC Recommend dilution:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">30-150</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">WB Predicted band size:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">68 kDa</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">WB Positive control:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">A375 and Jurkat cell lysates</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="201" valign="center" style="width: 150.85pt; padding: 0pt 3.75pt; border-left: 1pt dotted rgb(193, 193, 193); border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><b><span class="15" style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">WB Recommended dilution:</span></b><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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<td width="362" valign="center" style="width: 271.95pt; padding: 0pt 3.75pt; border-left: none; border-right: 1pt dotted rgb(193, 193, 193); border-top: none; border-bottom: 1pt dotted rgb(193, 193, 193); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="MsoNormal" style="margin: 0pt; text-indent: 0pt; word-break: break-all; line-height: 15pt;"><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;">500-2000</span><span style="font-family: 微软雅黑; color: rgb(34, 34, 34); letter-spacing: 0pt; font-size: 9pt;"><o:p></o:p></span></p>
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</table>
<p> </p>
<p><img src="/images/upload/Image/兔抗SLC3A2多克隆抗体1.jpg" width="600" height="450" alt="" /><br />
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<p><img src="/images/upload/Image/兔抗SLC3A2多克隆抗体.jpg" width="600" height="450" alt="" /><br />
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<p><img src="/images/upload/Image/兔抗SLC3A2多克隆抗体(1).jpg" width="271" height="450" alt="" /></p>
上海酶联生物抗体常见问题汇总
1. 收到抗体产品后应如何处理
收到抗体后,在分装储存及使用时需在生物安全柜中进行,并使用无菌枪头、离心管、注射器和缓冲液等,降低污染率。另外,使用前抗体要处于低温状态。
2. 如何正确保存抗体抗体产品
请将抗体置于4℃避光储存。无菌操作。即使分装后,也不推荐将抗体冷冻保存。避免将抗体稀释至工作浓度后在4℃条件下储存超过一天,建议现用现配。
3. 如果没按说明书上的储存要求,将抗体置于室温储存了一段时间,还可以继续使用
一般来说,室温下放置几个小时不会影响抗体的活性,但是放置几天或者更长时间则有可能会导致蛋白降解,影响抗体活性,不建议使用。
4. 抗体抗体的保质期有多久
按说明书要求储存,抗体抗体有效期为1年。
5. 抗体抗体是冻干粉还是液体形式
抗体抗体均以无菌PBS缓冲液的形式提供。该溶液中不含稳定剂、防腐剂、载体蛋白、甘油或任何其他添加剂。抗体的pI值决定了PBS缓冲液的pH值。
6. 抗体收到后有漂浮物、絮状沉淀或聚集物是否正常?应如何处理
存在聚集物或沉淀物属于正常现象,不会影响抗体的活性。可以在室温下轻轻摇晃使其溶解,或过滤或离心去除。
7. 抗体抗体的浓度是多少
抗体不同批次抗体的浓度不同,但一般来说是在4到11mg/mL之间。包装的离心管上会标记抗体的具体浓度。如果需要在订购之前知道产品的确切浓度,如果不能确定可以先联系我们。
8. 应如何稀释抗体至工作浓度
在没有InVivoPure稀释缓冲液的情况下,可以使用与抗体PBS缓冲液pH相同或相近的的无菌PBS,以避免蛋白聚集。稀释时,建议使用预冷缓冲液,并在生物安全柜中操作,使用无菌枪头、试管、注射器和缓冲液来保持抗体的无菌性。应避免将抗体稀释至工作浓度后在4℃条件下储存超过一天。
9. 抗体抗体的氨基酸或DNA序列是什么
我们的抗体是使用标准杂交瘤技术生产的,因此抗体的AA或DNA序列通常是未知的。
10. 抗体抗体的抗原表位是什么
抗体不提供抗原表位信息,请客户咨询上海酶联生物。
11. 抗体抗体在体内的半衰期是多久
厂家内部并没有抗体在体内的半衰期数据。因为抗体的半衰期是高度可变的,并且受到物种、同型、抗原分布、抗原浓度和许多其他因素的影响。参考与您实验条件及设计相近的实验数据,预估抗体的半衰期。
12. 抗体抗体是否适用于体内注射实验
抗体所有的产品都是专门为体内使用而配制的。所有抗体均在无菌 InVivoPure缓冲液中配制。抗体溶液不含稳定剂、防腐剂、载体蛋白、甘油或任何其他添加剂。内毒素水平 <2EU/mg ( InVivoMab),这使它们有资格进行大多数体内研究。为了满足最严格的要求,抗体还提供内毒素水平较低的抗体:<1EU/mg ( InVivoPlus)。
13. 抗体抗体是否适合体外应用
可用于体外应用,例如细胞培养实验和诊断测定,包括蛋白质印迹、免疫沉淀、IHC、流式细胞术等。请参阅各个产品页面以获取已批准的列表每种抗体的应用。另外所有抗体抗体都是未偶联的,因此流式细胞术和免疫荧光等应用需要使用荧光染料偶联的二抗。
14. 小老鼠体内注射实验,应该多久注射一次?每次注射多少抗体
任何给定实验和抗体的最佳给药剂量和频率可能会因实验系统(即小鼠品系、疾病模型等)、实验持续时间、靶组织、抗原浓度和许多其他因素而有很大差异细节。出于这个原因,最佳剂量最好通过阅读与您感兴趣的抗体相关的产品参考文献来确定,以查找与您的实验系统类似的实验系统中的已发布数据。以此现有研究为起点,您可以通过实验优化给药剂量和频率。抗体的每种产品在产品网页上都有最新的参考列表。
15. 体内实验中同种型对照组是否重要?如何选择合适的同种型对照
考虑同型对照处理组需要生成可靠的数据,因为它允许研究人员准确地区分以抗原特异性方式从初级抗体结合观察到的结果和从非抗原特异性结合或抗体注射的其他非特异性作用观察到的结果。同型对照抗体必须与一抗的宿主物种、同种型和亚类相匹配。为了方便我们的客户,我们在每个产品页面上列出了每种抗体的推荐的同型对照货号。
16. 如果觉得自己的抗体效果不佳,怎么办
如果您怀疑您的抗体没有发挥最佳功能,请务必认真填写并提交技术服务申请表。您填写的实验信息越充分,抗体技术团队的建议才更可靠。